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chemiluminescence autoradiography ecl kit sc 2048 santa cruz biotechnology  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology chemiluminescence autoradiography ecl kit sc 2048 santa cruz biotechnology
    Chemiluminescence Autoradiography Ecl Kit Sc 2048 Santa Cruz Biotechnology, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 3233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via <t>western</t> <t>blot</t> assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation <t>images</t> of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .
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    Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via <t>western</t> <t>blot</t> assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation <t>images</t> of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .
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    Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via <t>western</t> <t>blot</t> assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation <t>images</t> of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .
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    Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via <t>western</t> <t>blot</t> assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation <t>images</t> of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .
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    Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via western blot assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation images of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .

    Journal: iScience

    Article Title: Uncovering metabolic reprogramming in ovarian and cervical cancers with multi-omics

    doi: 10.1016/j.isci.2026.115834

    Figure Lengend Snippet: Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via western blot assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation images of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .

    Article Snippet: • Original western blot images have been deposited at Mendeley (Mendeley Data: https://data.mendeley.com/datasets/wv839nvbf8/1 ) and are publicly available.

    Techniques: Expressing, Knockdown, Control, Quantitative RT-PCR, Western Blot, MTT Assay, Colony Assay